Flow-sorted ram spermatozoa are highly susceptible to hydrogen peroxide damage but are protected by seminal plasma and catalase
T Leahy, P Celi, R Bathgate, G Evans, WMC Maxwell, JI Marti
Reproduction, Fertility and Development | CSIRO PUBLISHING | Published : 2010
To determine whether flow sorting increased the susceptibility of spermatozoa to reactive oxygen species (ROS), ram semen was either diluted with Tris medium (100 x 10(6) spermatozoa mL(-1); D) or highly diluted (10(6) spermatozoa mL(-1)) before being centrifuged (DC) at 750g for 7.5 min at 21 degrees C or flow-sorted (S) before cryopreservation. Thawed spermatozoa were resuspended in graded concentrations of hydrogen peroxide to induce oxidative stress. In Experiment 1, following exposure to 30 or 45 muM hydrogen peroxide (H(2)O(2)), the total motility (%) of DC (41.0 +/- 7.3 or 25.7 +/- 6.7, respectively) and S spermatozoa (33.8 +/- 6.3 or 20.1 +/- 6.3, respectively) was lower (P < 0.001) ..View full abstract
Awarded by Spanish Grant
The authors are grateful to Sexing Technologies (Navasota, TX, USA), the Spanish Grant PR2006-0186 for supporting this research and a scholarship from Australian Wool Innovation (to T.L.). The authors thank R. Leahy, K. Peake, K. Heasman, N. Satake and A. Souter for technical assistance.