An in vitro testing strategy towards mimicking the inhalation of high aspect ratio nanoparticles
Carola Endes, Otmar Schmid, Calum Kinnear, Silvana Mueller, Sandra Camarero-Espinosa, Dimitri Vanhecke, E Johan Foster, Alke Petri-Fink, Barbara Rothen-Rutishauser, Christoph Weder, Martin JD Clift
PARTICLE AND FIBRE TOXICOLOGY | BMC | Published : 2014
BACKGROUND: The challenge remains to reliably mimic human exposure to high aspect ratio nanoparticles (HARN) via inhalation. Sophisticated, multi-cellular in vitro models are a particular advantageous solution to this issue, especially when considering the need to provide realistic and efficient alternatives to invasive animal experimentation for HARN hazard assessment. By incorporating a systematic test-bed of material characterisation techniques, a specific air-liquid cell exposure system with real-time monitoring of the cell-delivered HARN dose in addition to key biochemical endpoints, here we demonstrate a successful approach towards investigation of the hazard of HARN aerosols in vitro...View full abstract
Awarded by Swiss National Science Foundation (National Research Programme 64)
MJDC would like to thank Dr. Craig Poland from the Institute of Occupational Medicine (IOM), Edinburgh, UK for kindly supplying the DQ12 sample. The LFA sample was also a kind gift to MJDC from Dr. C. A. Poland (IOM, Edinburgh) and Dr. Rodger Duffin (University of Edinburgh). This work was financially supported by the Swiss National Science Foundation (National Research Programme 64, Project #406440_131264/1) and the Adolphe Merkle Foundation.