Journal article

Surface charge of polymer coated SPIONs influences the serum protein adsorption, colloidal stability and subsequent cell interaction in vitro

Vera Hirsch, Calum Kinnear, Marc Moniatte, Barbara Rothen-Rutishauser, Martin JD Clift, Alke Fink



It is known that the nanoparticle-cell interaction strongly depends on the physicochemical properties of the investigated particles. In addition, medium density and viscosity influence the colloidal behaviour of nanoparticles. Here, we show how nanoparticle-protein interactions are related to the particular physicochemical characteristics of the particles, such as their colloidal stability, and how this significantly influences the subsequent nanoparticle-cell interaction in vitro. Therefore, different surface charged superparamagnetic iron oxide nanoparticles were synthesized and characterized. Similar adsorbed protein profiles were identified following incubation in supplemented cell cultu..

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University of Melbourne Researchers


Awarded by Swiss National Science Foundation

Funding Acknowledgements

The authors would like to thank Diego Chiappe (Proteomic Core Facility of the Ecole polytechnique federale de Lausanne (EPFL, Switzerland)) for his help with the LC-MS/MS measurements. The authors also acknowledge the funding provided by the Swiss National Science Foundation (PP00P2-123373/1 and 122355), the Adolphe Merkle Foundation, the University of Fribourg and FriMat (Fribourg Center for Nanomaterials).