Journal article

Role of the Protein Corona Derived from Human Plasma in Cellular Interactions between Nanoporous Human Serum Albumin Particles and Endothelial Cells

Mikhail V Zyuzin, Yan Yan, Raimo Hartmann, Katelyn T Gause, Moritz Nazarenus, Jiwei Cui, Frank Caruso, Wolfgang J Parak

BIOCONJUGATE CHEMISTRY | AMER CHEMICAL SOC | Published : 2017

Abstract

The presence of a protein corona on various synthetic nanomaterials has been shown to strongly influence how they interact with cells. However, it is unclear if the protein corona also exists on protein particles, and if so, its role in particle-cell interactions. In this study, pure human serum albumin (HSA) particles were fabricated via mesoporous silica particle templating. Our data reveal that various serum proteins adsorbed on the particles, when exposed to human blood plasma, forming a corona. In human umbilical vein endothelial cells (HUVECs), the corona was shown to decrease particle binding to the cell membrane, increase the residence time of particles in early endosomes, and reduce..

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Grants

Awarded by DAAD


Awarded by German Research Society (DFG)


Awarded by Australian Research Council (ARC) Centre of Excellence in Convergent Bio-Nano Science and Technology


Awarded by ARC under the Australian Laureate Fellowship scheme


Funding Acknowledgements

This work has been supported by DAAD (grant 56265460 to FC and WJP) and the German Research Society (DFG grant PA 794/25-1 to WJP). This research was also conducted and funded by the Australian Research Council (ARC) Centre of Excellence in Convergent Bio-Nano Science and Technology (Project Number CE140100036) and funded by the ARC under the Australian Laureate Fellowship (FL120100030) scheme.