Journal article

When size matters: differences in demineralized bone matrix particles affect collagen structure, mesenchymal stem cell behavior, and osteogenic potential

B Dozza, IG Lesci, S Duchi, E Della Bella, L Martini, F Salamanna, M Falconi, S Cinotti, M Fini, E Lucarelli, D Donati

JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A | WILEY | Published : 2017

Abstract

Demineralized bone matrix (DBM) is a natural, collagen-based, osteoinductive biomaterial. Nevertheless, there are conflicting reports on the efficacy of this product. The purpose of this study was to evaluate whether DBM collagen structure is affected by particle size and can influence DBM cytocompatibility and osteoinductivity. Sheep cortical bone was ground and particles were divided in three fractions with different sizes, defined as large (L, 1-2 mm), medium (M, 0.5-1 mm), and small (S, <0.5 mm). After demineralization, the chemical-physical analysis clearly showed a particle size-dependent alteration in collagen structure, with DBM-M being altered but not as much as DBM-S. DBM-M display..

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Grants

Funding Acknowledgements

Research reported in this publication was supported by Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia Romagna "Bruno Ubertini" (IZSLER), Brescia, Italy. The authors wish to thank Dr. Marco Gambarotti for his precious help on histological analysis, Dr. Gabriella Teti for her assistance with scanning electron microscopy, Charlotte Mcilwaine Story and Emily Aharon for editorial assistance.