Detection of mesenchymal stem cells senescence by prelamin A accumulation at the nuclear level

Abstract

Background: Human mesenchymal stem cells (MSC), during in vitro expansion, undergo a progressive loss of proliferative potential that leads to the senescent state, associated with a reduction of their “medicinal” properties. This may hampers their efficacy in the treatment of injured tissues. Quality controls on MSC-based cell therapy products should include an assessment of the senescent state. However, a reliable and specific marker is still missing. From studies on lamin-associated disorders, has emerged the correlation between defective lamin A maturation and cellular senescence. Findings: Primary cultured hMSC lines (n = 3), were analyzed by immunostaining at different life-span stages ..