Journal article
In vitro biosafety profile evaluation of multipotent mesenchymal stem cells derived from the bone marrow of sarcoma patients
E Lucarelli, C Bellotti, M Mantelli, MA Avanzini, R Maccario, F Novara, G Arrigo, O Zuffardi, M Zuntini, M Pandolfi, L Sangiorgi, D Lisini, D Donati, S Duchi
Journal of Translational Medicine | BMC | Published : 2014
Abstract
Background: In osteosarcoma (OS) and most Ewing sarcoma (EWS) patients, the primary tumor originates in the bone. Although tumor resection surgery is commonly used to treat these diseases, it frequently leaves massive bone defects that are particularly difficult to be treated. Due to the therapeutic potential of mesenchymal stem cells (MSCs), OS and EWS patients could benefit from an autologous MSCs-based bone reconstruction. However, safety concerns regarding the in vitro expansion of bone marrow-derived MSCs have been raised. To investigate the possible oncogenic potential of MSCs from OS or EWS patients (MSC-SAR) after expansion, this study focused on a biosafety assessment of MSC-SAR obt..
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Awarded by Italian Ministry of Health
Funding Acknowledgements
The authors are grateful to Dr. Panagiota Dimopoulou and Jennifer Perugini, for editorial assistance, to Dr. Francesca Ponti for DNA sequencing analysis. Research was supported in part by Progetto FIRB-Accordi di programma 2010 COD. RBAP10447, Italian Ministry of Health (Project IOR-2006-422755).