Journal article
Protein kinase B/AKT isoform 2 drives migration of human mesenchymal stem cells
Z Bulj, S Duchi, A Bevilacqua, A Gherardi, B Dozza, F Piccinini, GA Mariani, E Lucarelli, S Giannini, D Donati, S Marmiroli
International Journal of Oncology | SPANDIDOS PUBL LTD | Published : 2013
Abstract
This study was designed to investigate the migratory behavior of adult human mesenchymal stem cells (MSC) and the underlying mechanism. Cell migration was assessed by transwell, wound healing and time-lapse in vivo motility assays. Pharmacological inhibitors were used to determine the potential mechanism responsible for cell migration and invasion. The tests that were implemented revealed that MSC were fairly migratory. Protein kinase B (AKT) was strongly activated at the basal level. Through our analyses we demonstrated that pharmacological inactivation of AKT2 but not AKT1 significantly decreased cell migration and invasion. Although preliminary, collectively our results indicate that AKT2..
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Awarded by Progetto MIUR-FIRB
Awarded by Italian Ministry of Health
Funding Acknowledgements
The authors are grateful to Dr Panagiota Dimopoulou for editorial assistance. Research was supported in part by Progetto MIUR-FIRB (accordi di programma 2010 RBAP1044), MIUR-FIRB (Human Proteome Net), by Italian MIUR-Programma di Ricerca di Rilevante Interesse Nazionale Prin 2008 and by Italian Ministry of Health (project IOR-2006-422755). SM acknowledges the 'International short term mobility program' from Italian CNR.