Structure–function relationships of protein–lipopeptide complexes and influence on immunogenicity

AR Wijayadikusumah; LC Sullivan; DC Jackson; BY Chua

Journal article

Structure–function relationships of protein–lipopeptide complexes and influence on immunogenicity

AR Wijayadikusumah, LC Sullivan, DC Jackson, BY Chua

Amino Acids | SPRINGER WIEN | Published : 2017

DOI: 10.1007/s00726-017-2466-6

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Abstract

The lipopeptide, R4Pam2Cys, associates electrostatically with soluble protein antigens and significantly enhances their ability to induce protective humoral and cell-mediated responses. We demonstrate that antibody titers elicited by the antigen ovalbumin (OVA) associated with R4Pam2Cys are higher than those elicited by OVA in the presence of alum and comparable to those elicited by OVA formulated with complete Freund’s adjuvant (CFA). The hierarchy of anti-OVA antibody avidities was CFA > R4Pam2Cys = alum. Each of the three adjuvants facilitated IgG class-switching with significantly more IgG1 elicited by OVA when formulated with R4Pam2Cys. The effects of substituting naturally occurring l-..

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University of Melbourne Researchers

Lucy Sullivan Author

David Jackson Author

Brendon Chua Author

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Funding Acknowledgements

ARW was in receipt of student support from Bio Farma Perseo, Bandung, Java, Indonesia during the course of this work. DCJ is recipient of research fellowship APP1042018 from the National Health and Medical Research Council of Australia.