Journal article

Herpes simplex virus type 2 induces rapid cell death and functional impairment of murine dendritic cells in vitro

CA Jones, M Fernandez, K Herc, L Bosnjak, M Miranda-Saksena, RA Boadle, A Cunningham

Journal of Virology | AMER SOC MICROBIOLOGY | Published : 2003


Dendritic cells (DC) are critical for stimulation of naive T cells. Little is known about the effect of herpes simplex virus type 2 (HSV-2) infection on DC structure or function or if the observed effects of HSV-1 on human DC are reproduced in murine DC. Here, we demonstrate that by 12 h postinfection, wild-type (wt) HSV-2 (186) abortively infected murine bone marrow-derived DC and induced early cell death compared to UV-inactivated HSV-2 or mock-infected DC. HSV-2-induced loss of DC viability was more rapid than that induced by HSV-1 and was due, in part, to apoptosis, as shown by TEM, caspase-3 activation, and terminal deoxynucleotidyl transferase-mediated dCTP biotin nick end labeling. HS..

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