Custom-made novel biomimetic composite scaffolds for the bone regenerative medicine

Abstract

The aim of this pilot study was to test a new customized biomimetic composite scaffolds to restore bone defects using CAD-CAM technology. The new biomaterial was synthesized using biomimetic mineralized collagen, and poly (ε-caprolactone) (PCL). The mold used in scaffolding, the surgical guide, and the bony plate used during the surgery, were rapid prototyped (CAD-CAM technology). Our in vitro results showed that sheep mesenchymal stem cells (MSC) seeding ef ficiency was very high (>94%). Furthermore, cells can adhere, grow and maintain cell viability up to 7 days of in vitro cultivation on the scaffold as revealed by SEM and confocal laser scanning microscope analysis. These results are ind..