Minim Typing - A Rapid and Low Cost MLST Based Typing Tool for Klebsiella pneumoniae
Patiyan Andersson, Steven YC Tong, Jan M Bell, John D Turnidge, Philip M Giffard
PLOS ONE | PUBLIC LIBRARY SCIENCE | Published : 2012
Here we report a single nucleotide polymorphism (SNP) based genotyping method for Klebsiella pneumoniae utilising high-resolution melting (HRM) analysis of fragments within the multilocus sequence typing (MLST) loci. The approach is termed mini-MLST or Minim typing and it has previously been applied to Streptococcus pyogenes, Staphylococcus aureus and Enterococcus faecium. Six SNPs were derived from concatenated MLST sequences on the basis of maximisation of the Simpsons Index of Diversity (D). DNA fragments incorporating these SNPs and predicted to be suitable for HRM analysis were designed. Using the assumption that HRM alleles are defined by G+C content, Minim typing using six fragments w..View full abstract
Awarded by Australian National Health and Medical Research Council
This study was supported by project grant 390121 from the Australian National Health and Medical Research Council. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.