Journal article

Microbiological applications of high-resolution melting analysis

SYC Tong, PM Giffard

Journal of Clinical Microbiology | AMER SOC MICROBIOLOGY | Published : 2012

Abstract

High-resolution melting (HRM) analysis uses real-time PCR instrumentation to interrogate DNA sequence variation and is a low-cost, single-step, closed-tube method. Here we describe HRM technology and provide examples of varied clinical microbiological applications to highlight the strengths and limitations of HRM analysis. Copyright © 2012, American Society for Microbiology. All Rights Reserved.

University of Melbourne Researchers