Journal article
Rapid detection of H and R Panton-Valentine leukocidin isoforms in Staphylococcus aureus by high-resolution melting analysis
SYC Tong, RA Lilliebridge, DC Holt, GW Coombs, BJ Currie, PM Giffard
Diagnostic Microbiology and Infectious Disease | ELSEVIER SCIENCE INC | Published : 2010
Abstract
We designed a single-step, closed-tube, real-time polymerase chain reaction and high-resolution melting assay to simultaneously detect the presence of the Panton-Valentine leukocidin gene and discriminate histidine and arginine isoforms. Of 223 Staphylococcus aureus isolates from northern Australia, isoforms clustered by clonal complex (CC). All CC93 isolates harbored the arginine isoform. © 2010.
Grants
Awarded by National Health and Medical Research Council
Funding Acknowledgements
ST was supported as an Australian National Health and Medical Research Council PhD scholar (NHMRC grant 436033).