Journal article
Analysis of blaSHV codon 238 and 240 allele mixtures using Sybr green high-resolution melting analysis
P Andersson, T Harris, SYC Tong, PM Giffard
Antimicrobial Agents and Chemotherapy | AMER SOC MICROBIOLOGY | Published : 2009
DOI: 10.1128/AAC.00063-09
Abstract
Klebsiella pneumoniae isolates frequently contain complex mixtures of blaSHV alleles. A high-resolution melting-based method for interrogating the extended-spectrum activity conferring codon 238 and 240 polymorphisms was developed. This detects minority extended-spectrum β-lactamase-encoding alleles, allows estimation of allele ratios, and discriminates between single and double mutants. Copyright © 2009, American Society for Microbiology. All Rights Reserved.
Grants
Awarded by Australian National Health and Medical Research Council
Funding Acknowledgements
[ "This study was supported by project grant 390121 from the Australian National Health and Medical Research Council.", "We thank John Turnidge and the SENTRY program for the SENTRY isolates and Graeme Nimmo and Queensland Health Pathology Service for the PAH isolates." ]