Journal article

Functional analysis of the first complete genome sequence of a multidrug resistant sequence type 2 Staphylococcus epidermidis

Jean YH Lee, Ian R Monk, Sacha J Pidot, Siddarth Singh, Kyra YL Chua, Torsten Seemann, Timothy P Stinear, Benjamin P Howden



Staphylococcus epidermidis is a significant opportunistic pathogen of humans. The ST2 lineage is frequently multidrug-resistant and accounts for most of the clinical disease worldwide. However, there are no publically available, closed ST2 genomes and pathogenesis studies have not focused on these strains. We report the complete genome and methylome of BPH0662, a multidrug-resistant, hospital-adapted, ST2 S. epidermidis, and describe the correlation between resistome and phenotype, as well as demonstrate its relationship to publically available, international ST2 isolates. Furthermore, we delineate the methylome determined by the two type I restriction modification systems present in BPH0662..

View full abstract


Awarded by National Health and Medical Research Council, Australia (NHMRC)

Funding Acknowledgements

This project was supported by: the Royal Australasian College of Physicians, Basser Research Entry Scholarship to J. Y. H. L.; National Health and Medical Research Council, Australia (NHMRC) Project Grant (GNT1066791), and fellowships to B. P. H. (GNT1105905) and T. P. S. (GNT1008549). Doherty Applied Microbial Genomics is funded by The Department of Microbiology and Immunology at The University of Melbourne.