Journal article

Interferon-gamma released from omental adipose tissue of insulin-resistant humans alters adipocyte phenotype and impairs response to insulin and adiponectin release

JM Wentworth, J-G Zhang, E Bandala-Sanchez, G Naselli, R Liu, M Ritchie, GK Smyth, PE O'Brien, LC Harrison



BACKGROUND: Inflammatory factors derived from adipose tissue have been implicated in mediating insulin resistance in obesity. We sought to identify these using explanted human adipose tissue exposed to innate and adaptive immune stimuli. METHODS: Subcutaneous and omental adipose tissue from obese, insulin-resistant donors was cultured in the presence of macrophage and T-cell stimuli, and the conditioned medium tested for its ability to inhibit insulin-stimulated glucose uptake into human Simpson-Golabi-Behmel Syndrome (SGBS) adipocytes. The nature of the inhibitory factor in conditioned medium was characterized physicochemically, inferred by gene microarray analysis and confirmed by antibody..

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Awarded by Australian National Health and Medical Research Council

Funding Acknowledgements

We arc grateful to the trial participants. This work was supported by the Australian National Health and Medical Research Council (Program Grant 1037321 and Fellowship 1080887 to LCH, Program Grant 1016647 to JGZ, Program Grant 1054618 and Fellowship 1058892 to GKS and CRE 1078106 Fellowship to JMW). This work was made possible through Victorian State Government Operational Infrastructure Support and Australian National Health and Medical Research Council Research Institute Infrastructure Support Scheme.