Journal article

D-methionine interferes with non-typeable Haemophilus influenzae peptidoglycan synthesis during growth and biofilm formation

Harriet Dawe, Evelin Berger, Carina Sihlbom, Elizabeth M Angus, Robert P Howlin, Jay R Laver, Marc Tebruegge, Luanne Hall-Stoodley, Paul Stoodley, Saul N Faust, Raymond N Allan



Non-typeable Haemophilus influenzae (NTHi) is an opportunistic pathogen that plays a major role in a number of respiratory tract infections, including otitis media, cystic fibrosis and chronic obstructive pulmonary disease. Biofilm formation has been implicated in both NTHi colonization and disease, and is responsible for the increased tolerance of this pathogen towards antibiotic treatment. Targeting metabolic pathways that are important in NTHi biofilm formation represents a potential strategy to combat this antibiotic recalcitrance. A previous investigation demonstrated increased expression of a putative d-methionine uptake protein following exposure of NTHi biofilms to the ubiquitous sig..

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Awarded by NAMRIP's EPSRC grant NAMRA, EPSRC, Network for Antimicrobial Action, 'Bridging the Gap' programme

Awarded by Engineering and Physical Sciences Research Council

Awarded by National Institute for Health Research

Funding Acknowledgements

We are grateful to the Network for AntiMicrobial Resistance and Infection Prevention (NAMRIP) for support, including pump-priming funding via NAMRIP's EPSRC grant NAMRA (EP/M027260/1), part of the EPSRC, Network for Antimicrobial Action, 'Bridging the Gap' programme. Research support was provided by the Southampton NIHR Wellcome Trust Clinical Research Facility (R. A. and S. N. F.). The funders had no role in study design, data collection and interpretation.