A rigorous method to enrich for exosomes from brain tissue
Laura J Vella, Benjamin J Scicluna, Lesley Cheng, Emma G Bawden, Colin L Masters, Ching-Seng Ang, Nicholas Willamson, Catriona McLean, Kevin J Barnham, Andrew F Hill
JOURNAL OF EXTRACELLULAR VESICLES | TAYLOR & FRANCIS LTD | Published : 2017
Extracellular vesicles, including exosomes, are released by all cells, including those of the nervous system. Capable of delivering lipid, protein and nucleic acids to both nearby and distal cells, exosomes have been hypothesized to play a role in progression of many diseases of the nervous system. To date, most analyses on the role of these vesicles in the healthy and diseased state have relied on studying vesicles from in vitro sources, such as conditioned cell culture media, or body fluids. Here we have taken a critical approach to the enrichment and characterization of exosomes from human frontal cortex. This method maintains the integrity of the vesicles and their cargo, and comprehensi..View full abstract
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Awarded by National Health and Medical Research Council
This work was supported by the National Health and Medical Research Council [GNT1041413]; National Health and Medical Research Council [GNT1002349]; National Health and Medical Research Council ; National Health and Medical Research Council ; National Health and Medical Research Council ; CRC for Mental Health; The University of Melbourne [MRS].