Journal article

Effects of polymyxin-B on TNF-alpha production in equine whole blood stimulated with three different bacterial toxins

JR Bauquier, BS Tennent-Brown, E Tudor, SR Bailey

JOURNAL OF VETERINARY PHARMACOLOGY AND THERAPEUTICS | WILEY | Published : 2018

Abstract

Polymyxin-B is used to treat equine systemic inflammation. Bacterial toxins other than lipopolysaccharide (LPS) contribute to systemic inflammation but the effects of polymyxin-B on these are poorly defined. Whole blood aliquots from six healthy horses diluted 1:1 with RPMI were incubated for 21 hr with 1 μg/ml of LPS, lipoteichoic acid (LTA) or peptidoglycan (PGN) in the presence of increasing concentrations of polymyxin-B (10-3000 μg/ml). A murine L929 fibroblast bioassay was used to measure TNF-α activity. Polymyxin-B significantly inhibited the effects of all three bacterial toxins. Analysis of variance showed the IC50 value for polymyxin-B for TNF-α inhibition caused by LTA (11.19 ± 2.8..

View full abstract