SEPARATION OF MOUSE BONE-MARROW CELLS USING WHEAT-GERM AGGLUTININ AFFINITY CHROMATOGRAPHY

Abstract

SummaryMouse bone marrow cells were fractionated on columns of wheat germ agglutinin‐Sepharose 6MB (WGA‐Sepharose) and conditions established for specific binding and cell enrichment in the eluted fractions. A small proportion (7%) of the cells applied did not bind to the column and 60% of these were lymphocyte‐like cells. Twice as many cells were eluted from the WGA‐Sepharose column using N‐acetyl‐D‐glucosamine (GlcNAc), and 80% of these cells were polymorphonuclear granulocytes and metamyelocytes. These cells were only released from the matrix in the presence of GlcNAc at high buffer flow rates (> 4 ml/min).Approximately 3 × 108 bone marrow cells bound to 1 ml of WGA‐Sepharose. At least 5 ..