Journal article

Tentacle Transcriptome and Venom Proteome of the Pacific Sea Nettle, Chrysaora fuscescens (Cnidaria: Scyphozoa)

Dalia Ponce, Diane L Brinkman, Jeremy Potriquet, Jason Mulvenna

TOXINS | MDPI | Published : 2016

Abstract

Jellyfish venoms are rich sources of toxins designed to capture prey or deter predators, but they can also elicit harmful effects in humans. In this study, an integrated transcriptomic and proteomic approach was used to identify putative toxins and their potential role in the venom of the scyphozoan jellyfish Chrysaora fuscescens. A de novo tentacle transcriptome, containing more than 23,000 contigs, was constructed and used in proteomic analysis of C. fuscescens venom to identify potential toxins. From a total of 163 proteins identified in the venom proteome, 27 were classified as putative toxins and grouped into six protein families: proteinases, venom allergens, C-type lectins, pore-formi..

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Grants

Funding Acknowledgements

Dalia Ponce was supported by the Melbourne International Fee Remission Scholarship and the Melbourne International Research Scholarship granted by the University of Melbourne, and the Scholarship for Postgraduate Studies from the National Council of Science and Technology from Mexico (CONACyT). This research project was co-funded by the Australian Venom Research Unit (The Struan Sutherland Trust), the University of Melbourne, the Australian Institute of Marine Science and the QIMR Berghofer Medical Research Institute. We thank Senior Aquarist, Sharyl Crossley, from the Tennessee Aquarium (Chattanooga, TN, USA) for jellyfish sampling, Dr. Karen Luna-Ramirez for bioinformatics support and Associate Professor Christine Wright for commenting on the manuscript. QIMR Berghofer Medical Research Institute and the Australian Institute of Marine Science contributed funds for covering the costs to publish in open access.