Journal article

Studies on the mechanism of action of isopropyl N-phenyl carbamate.

RA Coss, RA Bloodgood, DL Brower, JD Pickett-Heaps, JR MacIntosh

Exp Cell Res | Published : 1975


The binding of [14C]isopropyl N-phenyl carbamate (IPC) to microtubular protein isolated from chick brains, and the effect of isopropyl N-phenyl carbamate (IPC) on the in vitro reassembly of microtubules was investigated. While [14C]colchicine binds to microtubular protein, [14C]IPC does not. Concentrations from 1 × 10-4 M to 1 × 10-3 M IPC do not prevent in vitro repolymerization of microtubular protein. IPC (1 × 10-4 M) does not affect the rate of reassembly of microtubules. We conclude that IPC does not exert its effect through an interaction with microtubular protein; we suggest that IPC probably interacts with microtubule organizing centers. © 1975.

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