Mucosa-associated invariant T cells infiltrate hepatic metastases in patients with colorectal carcinoma but are rendered dysfunctional within and adjacent to tumor microenvironment
Christopher R Shaler, Mauro E Tun-Abraham, Anton I Skaro, Khashayarsha Khazaie, Alexandra J Corbett, Tina Mele, Roberto Hernandez-Alejandro, SM Mansour Haeryfar
CANCER IMMUNOLOGY IMMUNOTHERAPY | SPRINGER | Published : 2017
Mucosa-associated invariant T (MAIT) cells are innate-like T lymphocytes that are unusually abundant in the human liver, a common site of colorectal carcinoma (CRC) metastasis. However, whether they contribute to immune surveillance against colorectal liver metastasis (CRLM) is essentially unexplored. In addition, whether MAIT cell functions can be impacted by chemotherapy is unclear. These are important questions given MAIT cells' potent immunomodulatory and inflammatory properties. Herein, we examined the frequencies and functions of peripheral blood, healthy liver tissue, tumor-margin and tumor-infiltrating MAIT cells in 21 CRLM patients who received no chemotherapy, FOLFOX, or a combinat..View full abstract
Awarded by Canadian Institutes of Health Research (CIHR) operating Grant
Awarded by NIH
This work was funded by a Canadian Institutes of Health Research (CIHR) operating Grant (MOP-130465) to S.M. Mansour Haeryfar and by a Dean's Research Initiative Award from Schulich School of Medicine and Dentistry, Western University, to Roberto Hernandez-Alejandro and S.M. Mansour Haeryfar. Khashayarsha Khazaie is supported by grant R01CA160436 from NIH, and Christopher R. Shaler is a CIHR postdoctoral fellowship recipient. We thank members of the Haeryfar laboratory for helpful discussions, Delfina Mazzuca for production and purification of staphylococcal enterotoxin B, and Katie Bain for technical assistance with preparation of Klebsiella lysate.