Journal article

Dynamic Covalent Hydrogels for Triggered Cell Capture and Release

Fatemeh Karimi, Joe Collins, Daniel E Heath, Luke A Connal

Bioconjugate Chemistry | AMER CHEMICAL SOC | Published : 2017

Abstract

A dual-responsive, cell capture and release surface was prepared through the incorporation of phenylboronic acid (PBA) groups into an oxime-based polyethylene glycol (PEG) hydrogel. Owing to its PEG-like properties, the unfunctionalized hydrogel was nonfouling. The use of highly efficient oxime chemistry allows the incorporation of commercially available 3,5-diformylphenyl boronic acid into the hydrogel matrix. Thus, the surface properties of the hydrogel were modified to enable reversible cell capture and release. Boronic ester formation between PBA groups and cell surface carbohydrates enabled efficient cell capture at pH 6.8. An increase to pH 7.8 resulted in cell detachment. This capture..

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Grants

Funding Acknowledgements

This work was supported by the Victorian Endowment for Science Knowledge and Innovation (LAC). The authors thank the Research Training Program (RTP), the Endeavor IPRS, and the Australian Postgraduate Awards (APA) for providing financial support to this project. We thank the Particulate Fluids Processing Center (PFPC) and the Materials Characterization and Fabrication Platform (MCFP).