Journal article

A new holistic 3D non-invasive analysis of cellular distribution and motility on fibroin-alginate microcarriers using light sheet fluorescent microscopy

Serena Duchi, Filippo Piccinini, Michela Pierini, Alessandro Bevilacqua, Maria Luisa Torre, Enrico Lucarelli, Spartaco Santi



Cell interaction with biomaterials is one of the keystones to developing medical devices for tissue engineering applications. Biomaterials are the scaffolds that give three-dimensional support to the cells, and are vectors that deliver the cells to the injured tissue requiring repair. Features of biomaterials can influence the behaviour of the cells and consequently the efficacy of the tissue-engineered product. The adhesion, distribution and motility of the seeded cells onto the scaffold represent key aspects, and must be evaluated in vitro during the product development, especially when the efficacy of a specific tissue-engineered product depends on viable and functional cell loading. In t..

View full abstract


Awarded by European Molecular Biology Organization (EM BO, Heidelberg, Germany)

Awarded by Associazione Italiana per la Ricerca sul Cancro-AIRC

Funding Acknowledgements

Dr. Spartaco Santi was supported by grants from the Ministero della Salute: "Conto Capitale 2013" Rizzoli Orthopaedic Institute; Dr. Filippo Piccinini was supported by a European Molecular Biology Organization (EM BO, Heidelberg, Germany) short term fellowship (EMBO ASTF 233-2015). The authors gratefully acknowledge funding from the Associazione Italiana per la Ricerca sul Cancro-AIRC, grant "MFAG-16491", P.I. Serena Duchi and support from "5 per mille 2014" - Rizzoli Orthopaedic Institute. The funders had no role in this study.