Journal article

Structured illumination microscopy reveals actin I localization in discreet foci in Plasmodium berghei gametocytes

Chiara Curra, Paul J McMillan, Lefteris Spanos, Vanessa Mollard, Elena Deligianni, Geoffrey McFadden, Leann Tilley, Inga Siden-Kiamos

EXPERIMENTAL PARASITOLOGY | ACADEMIC PRESS INC ELSEVIER SCIENCE | Published : 2017

Abstract

Actin has important roles in Plasmodium parasites but its exact function in different life stages is not yet fully elucidated. Here we report the localization of ubiquitous actin I in gametocytes of the rodent model parasite P. berghei. Using an antibody specifically recognizing F-actin and deconvolution microscopy we detected actin I in a punctate pattern in gametocytes. 3D-Structured Illumination Microscopy which allows sub-diffraction limit imaging resolved the signal into structures of less than 130 nm length. A portion of actin I was soluble, but the protein was also found complexed in a stabilized form which could only be completely solubilized by treatment with SDS. An additional popu..

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Grants

Awarded by I-MOVE Fellowship Programme


Awarded by General Secretariat for Research and Technology, Ministry of Education, Greece


Awarded by Australian National Health & Medical Research Council


Funding Acknowledgements

CC was the recipient of a fellowship from the I-MOVE Fellowship Programme - PCOFUND-GA-2010-267332 (Marie Curie). ISK is grateful for the generous funding from OzMalNet. This work was performed in the framework of the BIOSYS research project, Action KRIPIS, project No MIS-448301 (2013SE01380036) funded by the General Secretariat for Research and Technology, Ministry of Education, Greece and the European Regional Development Fund (Sectoral Operational Programme: Competitiveness and Entrepreneurship, NSRF 2007-2013)/European Commission. LT acknowledges funding from the Australian National Health & Medical Research Council (APP1098992).