Journal article

Structural and developmental expression of Ss-riok-2, an RIO protein kinase encoding gene of Strongyloides stercoralis

Wei-Qiang Lei, James B Lok, Wang Yuan, Yue-Zhou Zhang, Jonathan D Stoltzfus, Robin B Gasser, Si-Yuan He, Huan Zhou, Rui Zhou, Jun-Long Zhao, Min Hu

SCIENTIFIC REPORTS | NATURE RESEARCH | Published : 2017

Abstract

RIO kinases are essential atypical protein kinases in diverse prokaryotic and eukaryotic organisms, playing significant roles in yeast and humans. However, little is known about their functions in parasitic nematodes. In the present study, we have isolated and characterized the full-length cDNA, gDNA and a putative promoter of a RIOK-2 protein kinase (Ss-RIOK-2) encoding gene (Ss-riok-2) from Strongyloides stercoralis, a medically important parasitic nematode (Order Rhabditida). A three-dimensional structure (3D) model of Ss-RIOK-2 was generated using the Chaetomium thermophilum RIOK-2 protein kinase (Ct-RIOK-2) crystal structure 4GYG as a template. A docking study revealed some critical sit..

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University of Melbourne Researchers

Grants

Awarded by Fundamental Research Funds for the Central Universities


Awarded by National Key Basic Research Program (973 program) of China


Awarded by National Institute of Health (NIH), USA


Awarded by Huazhong Agricultural University Scientific & Technological Self-innovation Foundation


Awarded by NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES


Funding Acknowledgements

This work was supported by "Fundamental Research Funds for the Central Universities" (Program No. 2662015PY180) and "the National Key Basic Research Program (973 program) of China" (Grant No. 2015CB150300) to MH, by a grant from The National Institute of Health (NIH), USA (AI-50688) to JBL and by funds from Huazhong Agricultural University Scientific & Technological Self-innovation Foundation (Program No. 2015RC005) the National Health and Medical Research Council (NHMRC) and the Australian Research Council (ARC) of Australia to RBG.