Journal article
A novel Drosophila injury model reveals severed axons are cleared through a draper/MMP-1 signaling cascade
MD Purice, A Ray, EJ Münzel, BJ Pope, DJ Park, SD Speese, MA Logan
Elife | Published : 2017
DOI: 10.7554/eLife.23611
Abstract
Neural injury triggers swift responses from glia, including glial migration and phagocytic clearance of damaged neurons. The transcriptional programs governing these complex innate glial immune responses are still unclear. Here, we describe a novel injury assay in adult Drosophila that elicits widespread glial responses in the ventral nerve cord (VNC). We profiled injury-induced changes in VNC gene expression by RNA sequencing (RNA-seq) and found that responsive genes fall into diverse signaling classes. One factor, matrix metalloproteinase-1 (MMP-1), is induced in Drosophila ensheathing glia responding to severed axons. Interestingly, glial induction of MMP-1 requires the highly conserved e..
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Awarded by National Institute on Aging
Funding Acknowledgements
Glenn Foundation for Medical Research Glenn/AFAR Scholarship for Research in the Biology of Aging Maria D Purice National Institutes of Health NIH Neuroscience of Aging Training Grant T32AG023477 Maria D Purice National Institute of Neurological Disorders and Stroke R21 NS084112 Sean D Speese Mary A Logan Fred W. Fields Foundation New Investigator Award Sean D Speese Mary A Logan National Institute of Neurological Disorders and Stroke R01 NS079387-01 Mary A Logan Medical Research Foundation of Oregon New Investigator Grant Mary A Logan Ken and Ginger Harrison Term Professor Award Faculty Award Mary A Logan The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.