Journal article

Cytolytic DNA vaccine encoding lytic perforin augments the maturation of- and antigen presentation by- dendritic cells in a time-dependent manner

Danushka K Wijesundara, Wenbo Yu, Ben JC Quah, Preethi Eldi, John D Hayball, Kerrilyn R Diener, Ilia Voskoboinik, Eric J Gowans, Branka Grubor-Bauk

SCIENTIFIC REPORTS | NATURE PUBLISHING GROUP | Published : 2017

Abstract

The use of cost-effective vaccines capable of inducing robust CD8+ T cell immunity will contribute significantly towards the elimination of persistent viral infections and cancers worldwide. We have previously reported that a cytolytic DNA vaccine encoding an immunogen and a truncated mouse perforin (PRF) protein significantly augments anti-viral T cell (including CD8+ T cell) immunity. Thus, the current study investigated whether this vaccine enhances activation of dendritic cells (DCs) resulting in greater priming of CD8+ T cell immunity. In vitro data showed that transfection of HEK293T cells with the cytolytic DNA resulted in the release of lactate dehydrogenase, indicative of necrotic/l..

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Grants

Awarded by National Health and Medical Research Council (NHMRC) Australia


Awarded by Australian-Indian biotechnology fund


Funding Acknowledgements

Grants APP1026293, 543139 and 543143 from the National Health and Medical Research Council (NHMRC) Australia, grant BF040005 from the Australian-Indian biotechnology fund and a grant from the Hospital Research Foundation (THRF) supported the research in this study. THRF supports an early career fellowship for Danushka Wijesundara.