AMBRI biosensor - stabilizing artificial membranes and receptor attachment
CJ Burns, VBL Braach-Maksvytis, LG King, PDJ Osman, RJ Pace, B Raguse, L Wieczorek, BA Cornell
Proceedings of SPIE - The International Society for Optical Engineering | Published : 1999
The AMBRI Ion Channel Switch (ICS) biosensor is a novel sensing technology with broad application in a variety of fields. The technology is based on an artificial bilayer membrane attached to gold through hydrophilic tethers. The lamellar bilayer membrane possesses electrical characteristics similar to black (bilayer) lipid membranes being sealed and with a capacitance of approximately 0.6μF/cm2, is fluid, and is stable to a variety of media including plasma and whole blood and to challenges with solvent solutions. Receptors/antibodies can be attached to the membrane through biotin-streptavidin linkages, and use of caged biotin species allows optical patterning of the membrane surface.