Genetic influences determining progenitor cell mobilization and leukocytosis induced by granulocyte colony-stimulating factor

Abstract

The mechanisms involved in the mobilization of progenitor cells into the blood by granulocyte colony-stimulating factor (G-CSF) and other cytokines are poorly understood. To identify important influences on this complex process, in vive murine models were used. Granulocyte-macrophage colony stimulating factor (GM-CSF) transgenic, Max41 transgenic, W/W(v), Mpl-null, GM-CSF receptor (β chain)-null mice, wild-type littermate controls, and six inbred strains of mice were injected with 200 μg/kg/d G-CSF for 5 days. Three parameters of response were monitored: white blood cell count (WCC), peripheral blood progenitor cell (PBPC) numbers, and spleen weight. In all genotypes studied, G-CSF induced i..