The Characterisation of Pluripotent and Multipotent Stem Cells Using Fourier Transform Infrared Microspectroscopy
Julie Cao, Elizabeth S Ng, Donald McNaughton, Edouard G Stanley, Andrew G Elefanty, Mark J Tobin, Philip Heraud
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | MDPI | Published : 2013
Fourier transform infrared (FTIR) microspectroscopy shows potential as a benign, objective and rapid tool to screen pluripotent and multipotent stem cells for clinical use. It offers a new experimental approach that provides a holistic measurement of macromolecular composition such that a signature representing the internal cellular phenotype is obtained. The use of this technique therefore contributes information that is complementary to that acquired by conventional genetic and immunohistochemical methods.
Julie Cao was supported by the Australian Synchrotron Postgraduate Award. This work was supported by the Australian Research Council (ARC), Stem Cells Australia and the Australian National Health and Medical Research Council (NHMRC). AGE and EGS are Senior Research Fellows of the NHMRC.