APELIN promotes hematopoiesis from human embryonic stem cells
Qing C Yu, Claire E Hirst, Magdaline Costa, Elizabeth S Ng, Jacqueline V Schiesser, Karin Gertow, Edouard G Stanley, Andrew G Elefanty
BLOOD | AMER SOC HEMATOLOGY | Published : 2012
Transcriptional profiling of differentiating human embryonic stem cells (hESCs) revealed that MIXL1-positive mesodermal precursors were enriched for transcripts encoding the G-protein-coupled APELIN receptor (APLNR). APLNR-positive cells, identified by binding of the fluoresceinated peptide ligand, APELIN (APLN), or an anti-APLNR mAb, were found in both posterior mesoderm and anterior mesendoderm populations and were enriched in hemangioblast colony-forming cells (Bl-CFC). The addition of APLN peptide to the media enhanced the growth of embryoid bodies (EBs), increased the expression of hematoendothelial genes in differentiating hESCs, and increased the frequency of Bl-CFCs by up to 10-fold...View full abstract
This work was supported by grants from the Australian Stem Cell Center, Stem Cells Australia, the Juvenile Diabetes Research Foundation, and the National Health and Medical Research Council of Australia (NHMRC).