Journal article

APELIN promotes hematopoiesis from human embryonic stem cells

Qing C Yu, Claire E Hirst, Magdaline Costa, Elizabeth S Ng, Jacqueline V Schiesser, Karin Gertow, Edouard G Stanley, Andrew G Elefanty

BLOOD | AMER SOC HEMATOLOGY | Published : 2012

Abstract

Transcriptional profiling of differentiating human embryonic stem cells (hESCs) revealed that MIXL1-positive mesodermal precursors were enriched for transcripts encoding the G-protein-coupled APELIN receptor (APLNR). APLNR-positive cells, identified by binding of the fluoresceinated peptide ligand, APELIN (APLN), or an anti-APLNR mAb, were found in both posterior mesoderm and anterior mesendoderm populations and were enriched in hemangioblast colony-forming cells (Bl-CFC). The addition of APLN peptide to the media enhanced the growth of embryoid bodies (EBs), increased the expression of hematoendothelial genes in differentiating hESCs, and increased the frequency of Bl-CFCs by up to 10-fold...

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