Journal article
SIRPA, VCAM1 and CD34 identify discrete lineages during early human cardiovascular development
RJP Skelton, M Costa, DJ Anderson, F Bruveris, BW Finnin, K Koutsis, D Arasaratnam, AJ White, A Rafii, ES Ng, AG Elefanty, EG Stanley, CW Pouton, JM Haynes, R Ardehali, RP Davis, CL Mummery, DA Elliott
Stem Cell Research | ELSEVIER | Published : 2014
Abstract
The study of human cardiogenesis would benefit from a detailed cell lineage fate map akin to that established for the haematopoietic lineages. Here we sought to define cell lineage relationships based on the expression of NKX2-5 and the cell surface markers VCAM1, SIRPA and CD34 during human cardiovascular development. Expression of NKX2-5GFP was used to identify cardiac progenitors and cardiomyocytes generated during the differentiation of NKX2-5GFP/w human embryonic stem cells (hESCs). Cardiovascular cell lineages sub-fractionated on the basis of SIRPA, VCAM1 and CD34 expression were assayed for differentiation potential and gene expression. The NKX2-5posCD34pos population gave rise to end..
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Awarded by Department of Atomic Energy, Government of India
Funding Acknowledgements
We thank the Australian National Health and Medical Research Council (NHMRC) (DAE; 606586, Fellowship AGE, EGS) the NHMRC's Independent Research Institute Infrastructure Support Scheme, the Victorian Government's Operational Infrastructure Support Program, the Qatar National Research Foundation (MC, ART, AGE, EGS & DAE), JDRF (EGS & AGE) and the Netherlands Institute of Regenerative Medicine (RPD & CLM). Australian Post-graduate Awards (RJPS, BWF) and an EU Marie Curie International Outgoing Fellowship (DJA).