Derivation of endothelial cells from human embryonic stem cells in fully defined medium enables identification of lysophosphatidic acid and platelet activating factor as regulators of eNOS localization
Magdaline Costa, Koula Sourris, Sue Mei Lim, Qing C Yu, Claire E Hirst, Helena C Parkington, Vanta J Jokubaitis, Anthony E Dear, Hong B Liu, Suzanne J Micallef, Kathy Koutsis, Andrew G Elefanty, Edouard G Stanley
STEM CELL RESEARCH | ELSEVIER SCIENCE BV | Published : 2013
The limited availability of human vascular endothelial cells (ECs) hampers research into EC function whilst the lack of precisely defined culture conditions for this cell type presents problems for addressing basic questions surrounding EC physiology. We aimed to generate endothelial progenitors from human pluripotent stem cells to facilitate the study of human EC physiology, using a defined serum-free protocol. Human embryonic stem cells (hESC-ECs) differentiated under serum-free conditions generated CD34(+)KDR(+) endothelial progenitor cells after 6days that could be further expanded in the presence of vascular endothelial growth factor (VEGF). The resultant EC population expressed CD31 an..View full abstract
We thank Dr. S. Hawes and Dr. A. Pebay for their advice. This work was supported by the Australian Stem Cell Centre, Stem Cells Australia, The National Health and Medical Research council of Australia, The Juvenile Diabetes Research Foundation and The Australian Research Council.