Journal article

Functional Maturation of hPSC-Derived Forebrain Interneurons Requires an Extended Timeline and Mimics Human Neural Development

Cory R Nicholas, Jiadong Chen, Yunshuo Tang, Derek G Southwell, Nadine Chalmers, Daniel Vogt, Christine M Arnold, Ying-Jiun J Chen, Edouard G Stanley, Andrew G Elefanty, Yoshiki Sasai, Arturo Alvarez-Buylla, John LR Rubenstein, Arnold R Kriegstein

Cell Stem Cell | CELL PRESS | Published : 2013

Grants

Awarded by California Institute of Regenerative Medicine


Awarded by NATIONAL INSTITUTE OF DIABETES AND DIGESTIVE AND KIDNEY DISEASES


Awarded by NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES


Awarded by NATIONAL INSTITUTE OF NEUROLOGICAL DISORDERS AND STROKE


Funding Acknowledgements

We thank S. Baraban, R. Hunt, D. Wang, S. Gee, P. Parker, and M. Yang for electrophysiology guidance and discussion. We thank D. Hansen, J. Lui, M. Oldham, M. Paredes, C. Harwell, X. Wang, S. Wang, Y. Wang, S. Hart, R. Romero, T. Nguyen, and W. Walantus for helpful discussion, gene expression, cell culture, tissue procurement, processing, and technical assistance. We thank K. Probst for graphical design. We thank M. Ramalho-Santos and Y. Ohi for hiPSC lines, K. Deisseroth for the ChR2-EYFP plasmid, and K. Yoshikawa for the DLX2 antibody. We thank the staff and faculty at the San Francisco General Hospital Women's Options Center and at Advanced Bioscience Resources who assisted in human fetal tissue collection. This work was supported by the California Institute of Regenerative Medicine (RC1-00346 to A.R.K., RB2-01602 to J.L.R.R., and TR2-01749 to A.A.-B.), the National Institute of Neurological Disorders and Stroke, and the Osher Foundation. J.C. is a recipient of the CARE & CURE Pediatric Epilepsy Fellowship from Epilepsy Foundation of Greater Los Angeles. A.G.E. and E.G.S. are Senior Research Fellows of the National Health and Medical Research Council of Australia. C.R.N., A.A.-B., J.L.R.R., and A.R.K. are cofounders, board members, and shareholders of Neurona Therapeutics.