Journal article
Human chondrogenic paraxial mesoderm, directed specification and prospective isolation from pluripotent stem cells
K Umeda, J Zhao, P Simmons, E Stanley, A Elefanty, N Nakayama
Scientific Reports | NATURE PORTFOLIO | Published : 2012
DOI: 10.1038/srep00455
Abstract
Directed specification and prospective isolation of chondrogenic paraxial mesoderm progeny from human pluripotent stem (PS) cells have not yet been achieved. Here we report the successful generation of KDR- PDGFRα + progeny expressing paraxial mesoderm genes and the mesendoderm reporter MIXL1-GFP in a chemically defined medium containing the canonical WNT signaling activator, BMP-inhibitor, and the Nodal/Activin/ TGFÎ 2 signaling controller. Isolated (GFP +)KDR-PDGFRα + mesoderm cells were sensitive to sequential addition of the three chondrogenic factors PDGF, TGFβ and BMP. Under these conditions, the cells showed robust chondrogenic activity in micromass culture, and generated a hyaline-li..
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Awarded by Brown Foundation Institute of Molecular Medicine for the Prevention of Human Diseases
Funding Acknowledgements
First, we would like to acknowledge B. Davis and A. Crane for providing the HK1 hiPS cell line prior to publication. We also thank D. Havilland for cell sorting, and M. M. Jiang for histological analysis. This work was supported by Brown Foundation Institute of Molecular Medicine UTHSC (Startup Fund, NN), and Rolanette and Berdon Lawrence Bone Disease Program of Texas Research Award (NN).