Journal article
Productive infection of human embryonic stem cell-derived nkx2.1 respiratory progenitors with human rhinovirus
RA Jenny, C Hirst, SM Lim, AL Goulburn, SJ Micallef, T Labonne, A Kicic, KM Ling, SM Stick, ES Ng, A Trounson, A Giudice, AG Elefanty, EG Stanley
Stem Cells Translational Medicine | WILEY | Published : 2015
Abstract
Airway epithelial cells generated from pluripotent stem cells (PSCs) represent a resource for research into a variety of human respiratory conditions, including those resulting from infection with common human pathogens. Using an NKX2.1-GFP reporter humanembryonic stem cell line, wede-veloped a serum-free protocol for the generation of NKX2.1+ endoderm that, when transplanted into immunodeficient mice, matured into respiratory cell types identified by expression of CC10, MUC5AC, and surfactant proteins. Gene profiling experiments indicated that day 10 NKX2.1+ endo-derm expressed markers indicative of early foregut but lacked genes associated with later stages of respiratory epithelial cell d..
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Funding Acknowledgements
We thank Andrew Fryga and the staff of FlowCore (Monash University) for flow cytometric sorting. This work was supported by grants from the Australian Stem Cell Centre, Stem Cells Australia, the Juvenile Diabetes Research Foundation, the National Health and Medical Research Council of Australia (NHMRC), and the Victoria-California Stem Cell Alliance. A.G.E. and E.G.S. are Senior Research Fellows of the NHMRC. S.M.S is an NHMRC Practitioner Fellow.