CD13 and ROR2 Permit Isolation of Highly Enriched Cardiac Mesoderm from Differentiating Human Embryonic Stem Cells
Rhys JP Skelton, Bevin Brady, Suhail Khoja, Debashis Sahoo, James Engel, Deevina Arasaratnam, Kholoud K Saleh, Oscar J Abilez, Peng Zhao, Edouard G Stanley, Andrew G Elefanty, Murray Kwon, David A Elliott, Reza Ardehali
STEM CELL REPORTS | CELL PRESS | Published : 2016
The generation of tissue-specific cell types from human embryonic stem cells (hESCs) is critical for the development of future stem cell-based regenerative therapies. Here, we identify CD13 and ROR2 as cell-surface markers capable of selecting early cardiac mesoderm emerging during hESC differentiation. We demonstrate that the CD13+/ROR2+ population encompasses pre-cardiac mesoderm, which efficiently differentiates to all major cardiovascular lineages. We determined the engraftment potential of CD13+/ROR2+ in small (murine) and large (porcine) animal models, and demonstrated that CD13+/ROR2+ progenitors have the capacity to differentiate toward cardiomyocytes, fibroblasts, smooth muscle, and..View full abstract
Awarded by California Institute of Regenerative Medicine (CIRM)
Awarded by NATIONAL CANCER INSTITUTE
Awarded by NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES
The authors would like to thank all the members of the Ardehali laboratory for instructive discussions and suggestions. The authors would also like to thank the UCLA TRIC center and DLAM, namely Anthony Smithson, Sandra Duarte Vogel, and Janlee Jensen, for all their support and expertise in the handling of large animal models. Thanks are also due to the UCLA Flow Core, Namely Jessica Scholes and Felicia Codrea. Furthermore, the authors would like to especially thank Armin Hojjat, and Drs. Gay Crooks, Hanna Mikkola, and Thomas Vondriska for their valuable input and guidance. This work was supported in part by grants from the California Institute of Regenerative Medicine (CIRM) (RC1-00354-1) (R.A.) and Eli & Edith Broad Center of Regenerative Medicine and Stem Cell Research Center at UCLA Research Award (R.A.).