Journal article
Magnetic resonance imaging of iron oxide-labeled human embryonic stem cell-derived cardiac progenitors
RJP Skelton, S Khoja, S Almeida, S Rapacchi, F Han, J Engel, P Zhao, P Hu, EG Stanley, AG Elefanty, M Kwon, DA Elliott, R Ardehali
Stem Cells Translational Medicine | WILEY | Published : 2016
Abstract
Given the limited regenerative capacity of the heart, cellular therapy with stem cell-derived cardiac cells could be a potential treatment for patients with heart disease. However, reliable imaging techniques to longitudinally assess engraftment of the transplanted cells are scant. To address this issue, we used ferumoxytol as a labeling agent of human embryonic stem cell-derived cardiac progenitor cells (hESC-CPCs) to facilitate tracking by magnetic resonance imaging (MRI) in a large animal model. Differentiating hESCs were exposed to ferumoxytol at different time points and varying concentrations. We determined that treatment with ferumoxytol at 300 μg/ml on day 0 of cardiac differentiatio..
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Awarded by National Institute of General Medical Sciences
Funding Acknowledgements
We thank all the members of the Ardehali Laboratory for instructive discussions and suggestions. We also thank the University of California, Los Angeles (UCLA), Translational Research Imaging Center and Division of Laboratory Animal Medicine, especially Anthony Smithson, Sandra Duarte Vogel, and Janlee Jensen, for all their support and expertise in the handling of large animal models. Thanks are also due to the UCLA Flow Core, especially Jessica Scholes and Felicia Codrea. Furthermore, we especially thank Drs. Daniel Ennis, Kim Lien Nguyen, and Paul Finn for their valuable input and guidance. This work was supported in part by grants from the California Institute of Regenerative Medicine (CIRM) (Grant RC1-00354-1) to R.A. and the Eli & Edith Broad Center of Regenerative Medicine and Stem Cell Research Center at UCLA Research Award to R.A.