Journal article

Fourier transform infrared microspectroscopy identifies early lineage commitment in differentiating human embryonic stem cells

Philip Heraud, Elizabeth S Ng, Sally Caine, Qing C Yu, Claire Hirst, Robyn Mayberry, Amanda Bruce, Bayden R Wood, Don McNaughton, Edouard G Stanley, Andrew G Elefanty

STEM CELL RESEARCH | ELSEVIER SCIENCE BV | Published : 2010

Abstract

Human ESCs (hESCs) are a valuable tool for the study of early human development and represent a source of normal differentiated cells for pharmaceutical and biotechnology applications and ultimately for cell replacement therapies. For all applications, it will be necessary to develop assays to validate the efficacy of hESC differentiation. We explored the capacity for FTIR spectroscopy, a technique that rapidly characterises cellular macromolecular composition, to discriminate mesendoderm or ectoderm committed cells from undifferentiated hESCs. Distinct infrared spectroscopic "signatures" readily distinguished hESCs from these early differentiated progeny, with bioinformatic models able to c..

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