Fourier transform infrared microspectroscopy identifies early lineage commitment in differentiating human embryonic stem cells
Philip Heraud, Elizabeth S Ng, Sally Caine, Qing C Yu, Claire Hirst, Robyn Mayberry, Amanda Bruce, Bayden R Wood, Don McNaughton, Edouard G Stanley, Andrew G Elefanty
STEM CELL RESEARCH | ELSEVIER SCIENCE BV | Published : 2010
Human ESCs (hESCs) are a valuable tool for the study of early human development and represent a source of normal differentiated cells for pharmaceutical and biotechnology applications and ultimately for cell replacement therapies. For all applications, it will be necessary to develop assays to validate the efficacy of hESC differentiation. We explored the capacity for FTIR spectroscopy, a technique that rapidly characterises cellular macromolecular composition, to discriminate mesendoderm or ectoderm committed cells from undifferentiated hESCs. Distinct infrared spectroscopic "signatures" readily distinguished hESCs from these early differentiated progeny, with bioinformatic models able to c..View full abstract
This work was supported by the Australian Stem Cell Centre, the Juvenile Diabetes Research Foundation, and the National Health and Medical Research Council (NHMRC) of Australia. A.G.E. is a Senior Research Fellow of the NHMRC.