A protocol for removal of antibiotic resistance cassettes from human embryonic stem cells genetically modified by homologous recombination or transgenesis
Richard P Davis, Magdaline Costa, Catarina Grandela, Andrew M Holland, Tanya Hatzistavrou, Suzanne J Micallef, Xueling Li, Adam L Goulburn, Lisa Azzola, Andrew G Elefanty, Edouard G Stanley
Nature Protocols | NATURE PUBLISHING GROUP | Published : 2008
We thank Robyn Mayberry, Kathy Koutsis and Amanda Bruce for the provision of HESCs. This work was supported by the Australian Stem Cell Centre, the National Health and Medical Research Council of Australia (NHMRC) and the Juvenile Diabetes Research Foundation. A. G. E. is a senior research fellow of the NHMRC.