Journal article

Selective Manipulation of Discrete Mannosidase Activities in the Endoplasmic Reticulum by Using Reciprocally Selective Inhibitors

Taiki Kuribara, Makoto Hirano, Gaetano Speciale, Spencer J Williams, Yukishige Ito, Kiichiro Totani

CHEMBIOCHEM | WILEY-V C H VERLAG GMBH | Published : 2017

Abstract

Within the endoplasmic reticulum, immature glycoproteins are sorted into secretion and degradation pathways through the sequential trimming of mannose residues from Man9 GlcNAc2 to Man5 GlcNAc2 by the combined actions of assorted α-1,2-mannosidases. It has been speculated that specific glycoforms encode signals for secretion and degradation. However, it is unclear whether the specific signal glycoforms are produced by random mannosidase action or are produced regioselectively in a sequenced manner by specific α-1,2-mannosidases. Here, we report the identification of a set of selective mannosidase inhibitors and development of conditions for their use that enable production of distinct pools ..

View full abstract

University of Melbourne Researchers

Grants

Awarded by Japan Society for the Promotion of Science (JSPS) (KAKENHI)


Awarded by Australian Research Council


Awarded by Grants-in-Aid for Scientific Research


Funding Acknowledgements

This work was funded by the Japan Society for the Promotion of Science (JSPS) Grants-in-Aid for Scientific Research (KAKENHI) to K.T. (Nos. 25560420 and JP16K01938) and Y.I. (No. JP16H06290), a Discovery Project and Future Fellowship from the Australian Research Council to S.J.W. (Nos. DP160100597 and FT130100103,) and a JGC-S Foundation Scholarship to M.H.