Journal article
Gabapentin modulates HCN4 channel voltage-dependence
HS Tae, KM Smith, AM Phillips, KA Boyle, M Li, IC Forster, RJ Hatch, R Richardson, DI Hughes, BA Graham, S Petrou, CA Reid
Frontiers in Pharmacology | FRONTIERS MEDIA SA | Published : 2017
Open access
Abstract
Gabapentin (GBP) is widely used to treat epilepsy and neuropathic pain. There is evidence that GBP can act on hyperpolarization-activated cation (HCN) channel-mediated Ih in brain slice experiments. However, evidence showing that GBP directly modulates HCN channels is lacking. The effect of GBP was tested using two-electrode voltage clamp recordings from human HCN1, HCN2, and HCN4 channels expressed in Xenopus oocytes. Whole-cell recordings were also made from mouse spinal cord slices targeting either parvalbumin positive (PV+ ) or calretinin positive (CR+ ) inhibitory neurons. The effect of GBP on Ih was measured in each inhibitory neuron population. HCN4 expression was assessed in the spin..
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Awarded by Biotechnology and Biological Sciences Research Council
Funding Acknowledgements
This work was supported by National Health and Medical Research Council Program Grant (10915693) to SP and CR; National Health and Medical Research Council Project Grant (631000 and 631000) to BG; BBSRC Grant J000620/1 to DH; CR is supported by a Dowd Fellowship. The Florey Institute of Neuroscience and Mental Health is supported by Victorian State Government infrastructure funds.