Journal article

Gabapentin Modulates HCN4 Channel Voltage-Dependence

Han-Shen Tae, Kelly M Smith, A Marie Phillips, Kieran A Boyle, Melody Li, Ian C Forster, Robert J Hatch, Robert Richardson, David I Hughes, Brett A Graham, Steven Petrou, Christopher A Reid

FRONTIERS IN PHARMACOLOGY | FRONTIERS MEDIA SA | Published : 2017

Abstract

Gabapentin (GBP) is widely used to treat epilepsy and neuropathic pain. There is evidence that GBP can act on hyperpolarization-activated cation (HCN) channel-mediated Ih in brain slice experiments. However, evidence showing that GBP directly modulates HCN channels is lacking. The effect of GBP was tested using two-electrode voltage clamp recordings from human HCN1, HCN2, and HCN4 channels expressed in Xenopus oocytes. Whole-cell recordings were also made from mouse spinal cord slices targeting either parvalbumin positive (PV+) or calretinin positive (CR+) inhibitory neurons. The effect of GBP on Ih was measured in each inhibitory neuron population. HCN4 expression was assessed in the spinal..

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Grants

Awarded by National Health and Medical Research Council Program


Awarded by National Health and Medical Research Council Project Grant


Awarded by BBSRC Grant


Awarded by Biotechnology and Biological Sciences Research Council


Funding Acknowledgements

This work was supported by National Health and Medical Research Council Program Grant (10915693) to SP and CR; National Health and Medical Research Council Project Grant (631000 and 631000) to BG; BBSRC Grant J000620/1 to DH; CR is supported by a Dowd Fellowship. The Florey Institute of Neuroscience and Mental Health is supported by Victorian State Government infrastructure funds.