Journal article
Combined circulating tumor DNA and protein biomarker-based liquid biopsy for the earlier detection of pancreatic cancers
JD Cohen, AA Javed, C Thoburn, F Wong, J Tie, P Gibbs, CM Schmidt, MT Yip-Schneider, PJ Allen, M Schattner, RE Brand, AD Singhi, GM Petersen, SM Hong, SC Kim, M Falconi, C Doglioni, MJ Weiss, N Ahuja, J He Show all
Proceedings of the National Academy of Sciences of the United States of America | NATL ACAD SCIENCES | Published : 2017
Abstract
The earlier diagnosis of cancer is one of the keys to reducing cancer deaths in the future. Here we describe our efforts to develop a noninvasive blood test for the detection of pancreatic ductal adenocarcinoma. We combined blood tests for KRAS gene mutations with carefully thresholded protein biomarkers to determine whether the combination of these markers was superior to any single marker. The cohort tested included 221 patients with resectable pancreatic ductal adenocarcinomas and 182 control patients without known cancer. KRAS mutations were detected in the plasma of 66 patients (30%), and every mutation found in the plasma was identical to that subsequently found in the patient’s primar..
View full abstractGrants
Awarded by National Institutes of Health
Funding Acknowledgements
We thank C. Blair and K. Judge from The Ludwig Center at Johns Hopkins for expert technical and administrative assistance and Dr. Dae Wook Hwang, Department of Hepatobiliary and Pancreas Surgery, Asan Medical Center, University of Ulsan College of Medicine; J. Brooks, K. Chaffee, and W. Bamlet of the Mayo Clinic; and Dr. Herb Zeh of the University of Pittsburgh for their assistance with enrollment for this study. This work was supported by the Lustgarten Foundation for Pancreatic Cancer Research, The Virginia and D.K. Ludwig Fund for Cancer Research, The Conrad N. Hilton Foundation, The Sol Goldman Center for Pancreatic Cancer Research, The Michael Rolfe Pancreatic Cancer Research Foundation, The Joseph L. Rabinowitz Fund for Pancreatic Cancer Research, Susan Wojcicki and Dennis Troper, The Benjamin Baker Scholarship, The Commonwealth Foundation, National Health and Medical Research Council Grant APP1060804, The John Templeton Foundation, and NIH Grants P30-CA006973, P50-CA062924, P50-CA102701, CA06973, CA152753, CA196403, CA200468, GM-07309, and U01CA152753.