Journal article

Analysis of Prion Protein Conformation Using Circular Dichroism Spectroscopy

Laura J Ellett, Vanessa A Johanssen, VA Lawson (ed.)

PRIONS: METHODS AND PROTOCOLS | HUMANA PRESS INC | Published : 2017

Abstract

According to the protein-only hypothesis of prion propagation, the pathogenesis of prion disease is due to the misfolding of cellular PrP (PrPC) which gives rise to disease-associated PrPSc. This misfolding results in the predominantly α-helix secondary structure of PrP becoming increasingly β-sheet. Prion protein researchers often employ circular dichroism (CD) spectroscopy to rapidly analyze and identify the degree of α-helix and β-sheet content in their recombinant protein and peptide samples. CD is a nondestructive method of determining protein secondary structure and can be used to monitor the protein structural changes in various environments, e.g., pH and temperature. CD can also be u..

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University of Melbourne Researchers