Journal article
Generation of stable pluripotent stem cells from NOD mouse tail-tip fibroblasts
J Liu, MP Ashton, H Sumer, MK O'Bryan, TC Brodnicki, PJ Verma
Diabetes | AMER DIABETES ASSOC | Published : 2011
DOI: 10.2337/db10-1540
Open access
Abstract
OBJECTIVE - The NOD mouse strain has been widely used to investigate the pathology and genetic susceptibility for type 1 diabetes. Induced pluripotent stem cells (iPSCs) derived from this unique mouse strain would enable new strategies for investigating type 1 diabetes pathogenesis and potential therapeutic targets. The objective of this study was to determine whether somatic fibroblasts from NOD mice could be reprogrammed to become iPSCs, providing an alternative source of stem cells for the production of genetically modified NOD cells and mice. RESEARCH DESIGN AND METHODS - Adult tail-tip fibroblasts from male NOD mice were reprogrammed by retroviral transduction of the coding sequences of..
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Awarded by National Institute of Diabetes and Digestive and Kidney Diseases
Funding Acknowledgements
This work was funded in part by financial support from the Australian Phenomics Network, which is an initiative of the Australian Federal Government's National Collaborative Research Infrastructure Strategy; the Australian National Health and Medical Research Council (575552); and the National Institutes of Health/National Institute of Diabetes and Digestive and Kidney Diseases (5R01-DK-062882). This work was supported by the Victorian Government's Operational Infrastructure Support Program. M.K.O'B. is a National Health and Medical Research Council Fellow (No. 545805).