Journal article
RNA binding protein Musashi-2 regulates PIWIL1 and TBX1 in mouse spermatogenesis
Jessie M Sutherland, Alexander P Sobinoff, Barbara A Fraser, Kate A Redgrove, Nicole A Siddall, Peter Koopman, Gary R Hime, Eileen A McLaughlin
JOURNAL OF CELLULAR PHYSIOLOGY | WILEY | Published : 2018
DOI: 10.1002/jcp.26168
Abstract
RNA-binding proteins (RBP) are important facilitators of post-transcriptional gene regulation. We have previously established that nuclear overexpression of the RBP Musashi-2 (MSI2) during male germ cell maturation is detrimental to sperm cell development and fertility. Herein we determine the genes and pathways impacted by the upregulation of Msi2. Microarray analysis and qPCR confirmed differential gene expression in factors fundamental to the cell cycle, cellular proliferation, and cell death. Similarly, comparative protein expression analysis via iTRAQ, immunoblot, and immunolocalization, identified differential expression and localization of important regulators of transcription, transl..
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Funding Acknowledgements
Australian Research Council (ARC) Discovery Project; ARC Centre of Excellence in Biotechnology; Australian Postgraduate Award PhD Scholarship