Journal article
Recombinant expression and characterization of Lucilia cuprina CYP6G3: Activity and binding properties toward multiple pesticides
MJ Traylor, JM Baek, KE Richards, R Fusetto, W Huang, P Josh, Z Chen, P Bollapragada, RAJ O'Hair, P Batterham, EMJ Gillam
Insect Biochemistry and Molecular Biology | PERGAMON-ELSEVIER SCIENCE LTD | Published : 2017
Abstract
The Australian sheep blowfly, Lucilia cuprina, is a primary cause of sheep flystrike and a major agricultural pest. Cytochrome P450 enzymes have been implicated in the resistance of L. cuprina to several classes of insecticides. In particular, CYP6G3 is a L. cuprina homologue of Drosophila melanogaster CYP6G1, a P450 known to confer multi-pesticide resistance. To investigate the basis of resistance, a bicistronic Escherichia coli expression system was developed to co-express active L. cuprina CYP6G3 and house fly (Musca domestica) P450 reductase. Recombinant CYP6G3 showed activity towards the high-throughput screening substrates, 7-ethoxycoumarin and p-nitroanisole, but not towards p-nitroph..
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Funding Acknowledgements
Matthew Traylor's contribution was supported by a Fulbright Scholarship, funded by the Australian-American Fulbright Commission. Roberto Fusetto received scholarship support from the University of Melbourne. Financial support from the University of Melbourne Interdisciplinary Seed Grant program is gratefully acknowledged. We thank Dr. Phillip Daborn for his assistance in providing the cDNA for CYP6G3.